RRC ID 32478
Author Park JS, Pyo JH, Na HJ, Jeon HJ, Kim YS, Arking R, Yoo MA.
Title Increased centrosome amplification in aged stem cells of the Drosophila midgut.
Journal Biochem. Biophys. Res. Commun.
Abstract Age-related changes in long-lived tissue-resident stem cells may be tightly linked to aging and age-related diseases such as cancer. Centrosomes play key roles in cell proliferation, differentiation and migration. Supernumerary centrosomes are known to be an early event in tumorigenesis and senescence. However, the age-related changes of centrosome duplication in tissue-resident stem cells in vivo remain unknown. Here, using anti-γ-tubulin and anti-PH3, we analyzed mitotic intestinal stem cells with supernumerary centrosomes in the adult Drosophila midgut, which may be a versatile model system for stem cell biology. The results showed increased centrosome amplification in intestinal stem cells of aged and oxidatively stressed Drosophila midguts. Increased centrosome amplification was detected by overexpression of PVR, EGFR, and AKT in intestinal stem cells/enteroblasts, known to mimic age-related changes including hyperproliferation of intestinal stem cells and hyperplasia in the midgut. Our data show the first direct evidence for the age-related increase of centrosome amplification in intestinal stem cells and suggest that the Drosophila midgut is an excellent model for studying molecular mechanisms underlying centrosome amplification in aging adult stem cells in vivo.
Volume 450(2)
Pages 961-5
Published 2014-7-25
DOI 10.1016/j.bbrc.2014.06.085
PII S0006-291X(14)01159-0
PMID 24971546
MeSH Animals Cellular Senescence Centrosome / ultrastructure* Drosophila / cytology* Drosophila / metabolism Drosophila Proteins / metabolism Intestines / cytology Mitosis Oxidative Stress Proto-Oncogene Proteins c-akt / metabolism Receptor Protein-Tyrosine Kinases / metabolism Receptor, Epidermal Growth Factor / metabolism Receptors, Invertebrate Peptide / metabolism Stem Cells / ultrastructure*
IF 2.559
Times Cited 4
Drosophila esg-GAL4 UAS-GFP/CyO