Reference - Detail
| RRC ID | 32488 |
|---|---|
| Author | Bulat V, Rast M, Pielage J. |
| Title | Presynaptic CK2 promotes synapse organization and stability by targeting Ankyrin2. |
| Journal | J Cell Biol |
| Abstract |
The precise regulation of synapse maintenance is critical to the development and function of neuronal circuits. Using an in vivo RNAi screen targeting the Drosophila kinome and phosphatome, we identify 11 kinases and phosphatases controlling synapse stability by regulating cytoskeletal, phospholipid, or metabolic signaling. We focus on casein kinase 2 (CK2) and demonstrate that the regulatory (β) and catalytic (α) subunits of CK2 are essential for synapse maintenance. CK2α kinase activity is required in the presynaptic motoneuron, and its interaction with CK2β, mediated cooperatively by two N-terminal residues of CK2α, is essential for CK2 holoenzyme complex stability and function in vivo. Using genetic and biochemical approaches we identify Ankyrin2 as a key presynaptic target of CK2 to maintain synapse stability. In addition, CK2 activity controls the subcellular organization of individual synaptic release sites within the presynaptic nerve terminal. Our study identifies phosphorylation of structural synaptic components as a compelling mechanism to actively control the development and longevity of synaptic connections. |
| Volume | 204(1) |
| Pages | 77-94 |
| Published | 2014-1-6 |
| DOI | 10.1083/jcb.201305134 |
| PII | jcb.201305134 |
| PMID | 24395637 |
| PMC | PMC3882785 |
| MeSH | Animals Ankyrins / metabolism* Casein Kinase II / metabolism* Cytoskeleton / metabolism Drosophila Drosophila Proteins / metabolism* Phospholipids / metabolism Phosphoric Monoester Hydrolases / metabolism Phosphorylation Signal Transduction / physiology Synapses / metabolism Synapses / physiology* |
| IF | 8.811 |
| Times Cited | 16 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Blogs |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#141869 DGRC#141994 |