論文 - 詳細
| RRC ID | 32506 |
|---|---|
| 著者 | Huang J, Huang L, Chen YJ, Austin E, Devor CE, Roegiers F, Hong Y. |
| タイトル | Differential regulation of adherens junction dynamics during apical-basal polarization. |
| ジャーナル | J Cell Sci |
| Abstract |
Adherens junctions (AJs) in epithelial cells are constantly turning over to modulate adhesion properties under various physiological and developmental contexts, but how such AJ dynamics are regulated during the apical-basal polarization of primary epithelia remains unclear. Here, we used new and genetically validated GFP markers of Drosophila E-cadherin (DE-cadherin, hereafter referred to as DE-Cad) and β-catenin (Armadillo, Arm) to quantitatively assay the in vivo dynamics of biosynthetic turnover and membrane redistribution by fluorescence recovery after photobleaching (FRAP) assays. Our data showed that membrane DE-Cad and Arm in AJs of polarizing epithelial cells had much faster biosynthetic turnover than in polarized cells. Fast biosynthetic turnover of membrane DE-Cad is independent of actin- and dynamin-based trafficking, but is microtubule-dependent. Furthermore, Arm in AJs of polarizing cells showed a faster and diffusion-based membrane redistribution that was both quantitatively and qualitatively different from the slower and exchange-based DE-Cad membrane distribution, indicating that the association of Arm with DE-Cad is more dynamic in polarizing cells, and only becomes stable in polarized epithelial cells. Consistently, biochemical assays showed that the binding of Arm to DE-Cad is weaker in polarizing cells than in polarized cells. Our data revealed that the molecular interaction between DE-Cad and Arm is modulated during apical-basal polarization, suggesting a new mechanism that might be crucial for establishing apical-basal polarity through regulating the AJ dynamics. |
| 巻・号 | 124(Pt 23) |
| ページ | 4001-13 |
| 公開日 | 2011-12-1 |
| DOI | 10.1242/jcs.086694 |
| PII | jcs.086694 |
| PMID | 22159415 |
| PMC | PMC3244983 |
| MeSH | Adherens Junctions / chemistry Adherens Junctions / physiology* Animals Armadillo Domain Proteins / chemistry* Cadherins / chemistry* Cell Polarity* Drosophila / chemistry Drosophila / genetics Drosophila Proteins / chemistry* Embryo, Nonmammalian / chemistry Embryo, Nonmammalian / physiology Epithelial Cells / chemistry Epithelial Cells / cytology Epithelial Cells / physiology* Fluorescence Recovery After Photobleaching Green Fluorescent Proteins / chemistry Immunoprecipitation Membranes / chemistry Membranes / physiology Multiprotein Complexes / chemistry Protein Binding Protein Stability Protein Transport Transcription Factors / chemistry* |
| IF | 4.573 |
| 引用数 | 32 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | w ubi-DE-Cad::GFP |