RRC ID 32523
Author Harumoto T, Anbutsu H, Fukatsu T.
Title Male-killing Spiroplasma induces sex-specific cell death via host apoptotic pathway.
Journal PLoS Pathog.
Abstract Some symbiotic bacteria cause remarkable reproductive phenotypes like cytoplasmic incompatibility and male-killing in their host insects. Molecular and cellular mechanisms underlying these symbiont-induced reproductive pathologies are of great interest but poorly understood. In this study, Drosophila melanogaster and its native Spiroplasma symbiont strain MSRO were investigated as to how the host's molecular, cellular and morphogenetic pathways are involved in the symbiont-induced male-killing during embryogenesis. TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) staining, anti-cleaved-Caspase-3 antibody staining, and apoptosis-deficient mutant analysis unequivocally demonstrated that the host's apoptotic pathway is involved in Spiroplasma-induced male-specific embryonic cell death. Double-staining with TUNEL and an antibody recognizing epidermal marker showed that embryonic epithelium is the main target of Spiroplasma-induced male-specific apoptosis. Immunostaining with antibodies against markers of differentiated and precursor neural cells visualized severe neural defects specifically in Spiroplasma-infected male embryos as reported in previous studies. However, few TUNEL signals were detected in the degenerate nervous tissues of male embryos, and the Spiroplasma-induced neural defects in male embryos were not suppressed in an apoptosis-deficient host mutant. These results suggest the possibility that the apoptosis-dependent epidermal cell death and the apoptosis-independent neural malformation may represent different mechanisms underlying the Spiroplasma-induced male-killing. Despite the male-specific progressive embryonic abnormality, Spiroplasma titers remained almost constant throughout the observed stages of embryonic development and across male and female embryos. Strikingly, a few Spiroplasma-infected embryos exhibited gynandromorphism, wherein apoptotic cell death was restricted to male cells. These observations suggest that neither quantity nor proliferation of Spiroplasma cells but some Spiroplasma-derived factor(s) may be responsible for the expression of the male-killing phenotype.
Volume 10(2)
Pages e1003956
Published 2014-2
DOI 10.1371/journal.ppat.1003956
PII PPATHOGENS-D-13-02364
PMID 24550732
PMC PMC3923752
MeSH Animals Apoptosis Drosophila melanogaster / microbiology* Embryo, Nonmammalian / microbiology Female Immunohistochemistry In Situ Nick-End Labeling Male Reverse Transcriptase Polymerase Chain Reaction Spiroplasma* Symbiosis
IF 6.463
Times Cited 18
WOS Category PARASITOLOGY MICROBIOLOGY VIROLOGY
Resource
Drosophila Df(3L)H99 kni[ri-1] p[p]/TM3 Sb[1] (DGRC#106395)