RRC ID |
32533
|
Author |
Hiroi M, Ohkura M, Nakai J, Masuda N, Hashimoto K, Inoue K, Fiala A, Tabata T.
|
Title |
Principal component analysis of odor coding at the level of third-order olfactory neurons in Drosophila.
|
Journal |
Genes Cells
|
Abstract |
Olfactory information in Drosophila is conveyed by projection neurons from olfactory sensory neurons to Kenyon cells (KCs) in the mushroom body (MB). A subset of KCs responds to a given odor molecule, and the combination of these KCs represents a part of the neuronal olfactory code. KCs are also thought to function as coincidence detectors for memory formation, associating odor information with a coincident punishment or reward stimulus. Associative conditioning has been shown to modify KC output. This plasticity occurs in the vertical lobes of MBs containing α/α' branches of KCs, which is shown by measuring the average Ca(2+) levels in the branch of each lobe. We devised a method to quantitatively describe the population activity patterns recorded from axons of >1000 KCs at the α/α' branches using two-photon Ca(2+) imaging. Principal component analysis of the population activity patterns clearly differentiated the responses to distinct odors.
|
Volume |
18(12)
|
Pages |
1070-81
|
Published |
2013-12-1
|
DOI |
10.1111/gtc.12094
|
PMID |
24118654
|
MeSH |
Animals
Axons / physiology
Cyclohexanols / pharmacology
Drosophila / physiology*
Mushroom Bodies / cytology
Mushroom Bodies / physiology
Octanols / pharmacology
Odorants*
Olfactory Receptor Neurons / physiology*
Principal Component Analysis
Smell
|
IF |
1.655
|
Times Cited |
4
|
WOS Category
|
GENETICS & HEREDITY
CELL BIOLOGY
|
Resource |
Drosophila |
OK107 gal4 driver |