RRC ID 32543
Author You J, Zhang Y, Li Z, Lou Z, Jin L, Lin X.
Title Drosophila perlecan regulates intestinal stem cell activity via cell-matrix attachment.
Journal Stem Cell Reports
Abstract Stem cells require specialized local microenvironments, termed niches, for normal retention, proliferation, and multipotency. Niches are composed of cells together with their associated extracellular matrix (ECM). Currently, the roles of ECM in regulating niche functions are poorly understood. Here, we demonstrate that Perlecan (Pcan), a highly conserved ECM component, controls intestinal stem cell (ISC) activities and ISC-ECM attachment in Drosophila adult posterior midgut. Loss of Pcan from ISCs, but not other surrounding cells, causes ISCs to detach from underlying ECM, lose their identity, and fail to proliferate. These defects are not a result of a loss of epidermal growth factor receptor (EGFR) or Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling activity but partially depend on integrin signaling activity. We propose that Pcan secreted by ISCs confers niche properties to the adjacent ECM that is required for ISC maintenance of stem cell identity, activity, and anchorage to the niche.
Volume 2(6)
Pages 761-9
Published 2014-6-3
DOI 10.1016/j.stemcr.2014.04.007
PII S2213-6711(14)00114-3
PMID 24936464
PMC PMC4050351
MeSH Animals Drosophila Drosophila Proteins / metabolism* ErbB Receptors / metabolism Extracellular Matrix / metabolism* Heparan Sulfate Proteoglycans / metabolism* Intestines / cytology* Stem Cells / metabolism*
IF 6.032
Times Cited 20
WOS Category CELL & TISSUE ENGINEERING CELL BIOLOGY
Resource
Drosophila DGRC#111803 DGRC#111763