| RRC ID |
3255
|
| 著者 |
Tanaka S, Sugasawa H, Morii T, Okada T, Abe M, Kato N, Kuroda R, Nasu T, Nagai M, Umemura K.
|
| タイトル |
A new method of biosensing with 1 microl of Escherichia coli suspension using atomic force microscopy.
|
| ジャーナル |
Anal Biochem
|
| Abstract |
We developed a new method for detecting bacterial cells from 1-mul samples with atomic force microscopy (AFM). The use of a parafilm surface as a sample palette was effective for reacting small amounts of samples with an AFM probe. This was due to the parafilm's hydrophobic, semitransparent, and nonadhesive surface. In this way, all processes, such as the surface functionalization of a cantilever and the adhesion of Escherichia coli cells to a cantilever, were easily completed. In addition, we succeeded in detecting cell adsorption on the same AFM cantilever by both the drive mode and the thermal mode. The resonance frequency shift caused by cell adhesion was clearly detected by the two modes for the first time. Our data indicated the potential of applying AFM nanobiosensing to extremely small amounts of samples.
|
| 巻・号 |
345(1)
|
| ページ |
116-21
|
| 公開日 |
2005-10-1
|
| DOI |
10.1016/j.ab.2005.07.019
|
| PII |
S0003-2697(05)00530-0
|
| PMID |
16125129
|
| MeSH |
Bacterial Adhesion
Biosensing Techniques* / methods
Escherichia coli*
Microscopy, Atomic Force* / methods
Nanotechnology* / methods
Sample Size
Surface Properties
|
| IF |
2.877
|
| 引用数 |
2
|
|
WOS 分野
|
CHEMISTRY, ANALYTICAL
BIOCHEMICAL RESEARCH METHODS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
W3110 |
