論文 - 詳細
| RRC ID | 32588 |
|---|---|
| 著者 | Kayagaki N, Yamaguchi N, Nakayama M, Kawasaki A, Akiba H, Okumura K, Yagita H. |
| タイトル | Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity. |
| ジャーナル | J Immunol |
| Abstract |
TNF-related apoptosis-inducing ligand (TRAIL) has been identified as a member of the TNF family that induces apoptosis in a variety of tumor cells, but its physiological functions are largely unknown. In the present study, we examined the expression and function of TRAIL in human CD4+ T cell clones by utilizing newly established anti-human TRAIL mAbs. Human CD4+ T cell clones, HK12 and 4HM1, exhibited perforin-independent and Fas ligand (FasL)-independent cytotoxicity against certain target cells, including T lymphoma (Jurkat) and keratinocyte (HaCaT) cell lines, which are susceptible to TRAIL-mediated cytotoxicity. In contrast to FasL, the expression of which was inducible upon anti-CD3 stimulation, TRAIL was constitutively expressed on HK12 and 4HM1 cells, and no further increase was observed after anti-CD3 stimulation. Spontaneous cytotoxic activities of resting HK12 and 4HM1 cells against Jurkat and HaCaT cells were blocked by anti-TRAIL mAb but not by anti-FasL mAb, and bystander cytotoxic activities of anti-CD3-stimulated HK12 and 4HM1 cells were abolished by the combination of anti-TRAIL and anti-FasL mAbs. These results indicate a differential regulation of TRAIL and FasL expression on human CD4+ T cell clones and that TRAIL constitutes an additional pathway of T cell-mediated cytotoxicity. |
| 巻・号 | 162(5) |
| ページ | 2639-47 |
| 公開日 | 1999-3-1 |
| PMID | 10072506 |
| MeSH | Animals Antibodies, Monoclonal / immunology Apoptosis Regulatory Proteins CD4-Positive T-Lymphocytes / immunology* Cytotoxicity, Immunologic* Fas Ligand Protein Humans Membrane Glycoproteins / analysis Membrane Glycoproteins / physiology* Mice Perforin Pore Forming Cytotoxic Proteins Receptors, TNF-Related Apoptosis-Inducing Ligand Receptors, Tumor Necrosis Factor / physiology TNF-Related Apoptosis-Inducing Ligand Transfection Tumor Cells, Cultured Tumor Necrosis Factor-alpha / analysis Tumor Necrosis Factor-alpha / physiology* |
| IF | 4.886 |
| 引用数 | 203 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | hTRAIL/pMKITneo (RDB02934) |