RRC ID 32588
Author Kayagaki N, Yamaguchi N, Nakayama M, Kawasaki A, Akiba H, Okumura K, Yagita H.
Title Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity.
Journal J Immunol
Abstract TNF-related apoptosis-inducing ligand (TRAIL) has been identified as a member of the TNF family that induces apoptosis in a variety of tumor cells, but its physiological functions are largely unknown. In the present study, we examined the expression and function of TRAIL in human CD4+ T cell clones by utilizing newly established anti-human TRAIL mAbs. Human CD4+ T cell clones, HK12 and 4HM1, exhibited perforin-independent and Fas ligand (FasL)-independent cytotoxicity against certain target cells, including T lymphoma (Jurkat) and keratinocyte (HaCaT) cell lines, which are susceptible to TRAIL-mediated cytotoxicity. In contrast to FasL, the expression of which was inducible upon anti-CD3 stimulation, TRAIL was constitutively expressed on HK12 and 4HM1 cells, and no further increase was observed after anti-CD3 stimulation. Spontaneous cytotoxic activities of resting HK12 and 4HM1 cells against Jurkat and HaCaT cells were blocked by anti-TRAIL mAb but not by anti-FasL mAb, and bystander cytotoxic activities of anti-CD3-stimulated HK12 and 4HM1 cells were abolished by the combination of anti-TRAIL and anti-FasL mAbs. These results indicate a differential regulation of TRAIL and FasL expression on human CD4+ T cell clones and that TRAIL constitutes an additional pathway of T cell-mediated cytotoxicity.
Volume 162(5)
Pages 2639-47
Published 1999-3-1
PMID 10072506
MeSH Animals Antibodies, Monoclonal / immunology Apoptosis Regulatory Proteins CD4-Positive T-Lymphocytes / immunology* Cytotoxicity, Immunologic* Fas Ligand Protein Humans Membrane Glycoproteins / analysis Membrane Glycoproteins / physiology* Mice Perforin Pore Forming Cytotoxic Proteins Receptors, TNF-Related Apoptosis-Inducing Ligand Receptors, Tumor Necrosis Factor / physiology TNF-Related Apoptosis-Inducing Ligand Transfection Tumor Cells, Cultured Tumor Necrosis Factor-alpha / analysis Tumor Necrosis Factor-alpha / physiology*
IF 4.886
Times Cited 203
DNA material hTRAIL/pMKITneo (RDB02934)