論文 - 詳細
| RRC ID | 3260 |
|---|---|
| 著者 | Zhu J, Shimizu K. |
| タイトル | Effect of a single-gene knockout on the metabolic regulation in Escherichia coli for D-lactate production under microaerobic condition. |
| ジャーナル | Metab Eng |
| Abstract |
The effects of several single-gene knockout mutants (pykF, ppc, pflA, pta, and adhE mutants) on the metabolic flux distribution in Escherichia coli were investigated under microaerobic condition. The intracellular metabolite concentrations and enzyme activities were measured, and the metabolic flux distribution was computed to study the metabolic regulation in the cell. The pflA, pta and ppc mutants produced large amount of lactate when using glucose as a carbon source under microaerobic condition. Comparing the flux distribution and the enzyme activities in the mutants, it was shown that the lactate production was promoted by the inactivation of pyruvate formate lyase and the resulting overexpression of lactate dehydrogenase. The flux through Pta-Ack pathways and the ethanol production were limited by the available acetyl coenzyme A. It was shown that the glycolysis was activated in pykF mutant in microaerobic culture. The glycolytic flux was related with Pyk activity except for pykF mutant. The cell growth rate was shown to be affected by the flux through phosphoenolpyruvate carboxylase. The quantitative regulation analysis was made based on the deviation indexes. |
| 巻・号 | 7(2) |
| ページ | 104-15 |
| 公開日 | 2005-3-1 |
| DOI | 10.1016/j.ymben.2004.10.004 |
| PII | S1096-7176(04)00077-1 |
| PMID | 15781419 |
| MeSH | Aerobiosis / genetics Aerobiosis / physiology Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Gene Deletion* Gene Expression Regulation, Bacterial* Gene Expression Regulation, Enzymologic* Lactic Acid / biosynthesis* |
| IF | 7.263 |
| 引用数 | 75 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW0885 JW2294 JW3928 JW1228 JW1666 |