RRC ID 3262
著者 Adachi S, Hori K, Hiraga S.
タイトル Subcellular positioning of F plasmid mediated by dynamic localization of SopA and SopB.
ジャーナル J Mol Biol
Abstract SopA, SopB proteins and the cis-acting sopC DNA region of F plasmid are essential for partitioning of the plasmid, ensuring proper subcellular positioning of the plasmid DNA molecules. We have analyzed by immunofluorescence microscopy the subcellular localization of SopA and SopB. The majority of SopB molecules formed foci, which localized frequently with F plasmid DNA molecules. The foci increased in number in proportion to the cell length. Interestingly, beside the foci formation, SopB formed a spiral structure that was dependent on SopA, which also formed a spiral structure, independent of the presence of SopB, and these two structures partially overlapped. On the basis of these results and previous biochemical studies together with our simulations, we propose a theoretical model named "the reaction-diffusion partitioning model", using reaction-diffusion equations that explain the dynamic subcellular localization of SopA and SopB proteins and the subcellular positioning of F plasmid. We hypothesized that sister copies of plasmid DNA compete with each other for sites at which SopB multimer is at the optimum concentration. The plasmid incompatibility mediated by the Sop system might be explained clearly by this hypothesis.
巻・号 356(4)
ページ 850-63
公開日 2006-3-3
DOI 10.1016/j.jmb.2005.11.088
PII S0022-2836(05)01536-6
PMID 16403518
MeSH Animals Bacterial Proteins / genetics Bacterial Proteins / metabolism* Escherichia coli / cytology Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* F Factor / metabolism* Immunohistochemistry Mathematics Models, Theoretical Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Subcellular Fractions / chemistry
IF 4.76
引用数 61
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ?W3110