| RRC ID |
32620
|
| 著者 |
Sano A, Kurita N, Iabuki K, Coelho R, Takeo K, Nishimura K, Miyaji M.
|
| タイトル |
A comparative study of four different staining methods for estimation of live yeast form cells of Paracoccidioides brasiliensis.
|
| ジャーナル |
Mycopathologia
|
| Abstract |
A comparative study of four different staining methods for estimation of live yeast form cells of Paracoccidioides brasiliensis was carried out. The staining methods used were fluorescent staining, vital dye exclusion tests with erythrosin B and by Janus green and lactophenol cotton blue staining. Colony forming units (cfu) of the yeast form of eight P. brasiliensis isolates on brain heart infusion agar (BHIA) supplemented with 4% horse serum plus 5% P. brasiliensis cell extract (BHIA + HS + EXT) were examined for reliability of staining in determining the number of live fungal units in eight different isolates. Cfu on BHIA + HS + EXT plates showed an excellent plating efficiency over 96% in all isolates tested. The percentage of the live cells indicated by fluorescent staining (FL) or vital dye exclusion test with erythrosin B (EB) or Janus green (JG-1) was lower than that of cfu. By contrast, the percentage due to modified dye exclusion test with Janus green (JG-2) and that due to lactophenol cotton blue staining (LPCB) showed a close correration to that of cfu. Our results indicate that the modified dye exclusion test with Janus green and lactophenol cotton blue staining are useful for estimating cell viability of yeast form cells of P. brasiliensis.
|
| 巻・号 |
124(3)
|
| ページ |
157-61
|
| 公開日 |
1993-12-1
|
| DOI |
10.1007/BF01103733
|
| PMID |
7517514
|
| MeSH |
Azo Compounds
Benzenesulfonates*
Coloring Agents*
Erythrosine
Ethidium
Fluoresceins
Lactates*
Lactic Acid*
Mycology / methods*
Paracoccidioides / cytology*
Paracoccidioides / growth & development
Phenols*
Staining and Labeling / methods
|
| IF |
2.452
|
| 引用数 |
27
|
|
WOS 分野
|
MYCOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 41620
IFM 41621
IFM 41624
IFM 41626
IFM 41628
IFM 41632
IFM 41633
IFM 46463 |
