論文 - 詳細
| RRC ID | 32636 |
|---|---|
| 著者 | Hashiguchi M, Kashiwakura Y, Kojima H, Kobayashi A, Kanno Y, Kobata T. |
| タイトル | IL-33 activates eosinophils of visceral adipose tissue both directly and via innate lymphoid cells. |
| ジャーナル | Eur J Immunol |
| Abstract |
Eosinophils are multifunctional leukocytes involved in allergic reactions as well as adipose tissue regulation. IL-5 is required for eosinophil survival; however, the in vivo mechanisms of eosinophil regulation are not fully understood. A tg mouse model with il5 promoter-driven EGFP expression was established for detecting the IL-5-producing cells in vivo. Il5-egfp tg mice expressed high levels of EGFP in gonadal adipose tissue (GAT) cells. EGFP(+) cells in GAT were mainly group 2 innate lymphoid cells (ILCs). IL-33 preferentially expanded EGFP(+) cells and eosinophils in GAT in vivo. EGFP(+) ILCs were found to upregulate prg2 mRNA expression in GAT eosinophils. These results demonstrate that ILCs activate eosinophils in GAT. The blockage of IL-33Rα, on the other hand, did not impair EGFP(+) ILC numbers but did impair eosinophil numbers in vivo. GAT eosinophils expressed IL-33Rα and IL-33 expanded eosinophil numbers in CD90(+) cell-depleted mice. IL-33 was further observed to induce the expression of retnla and epx mRNA in eosinophils. These findings demonstrate that IL-33 directly activates eosinophils in GAT, and together with our other findings described above, our findings show that IL-33 has dual pathways via which it activates eosinophils in vivo: a direct activation pathway and a group 2 ILC-mediated pathway. |
| 巻・号 | 45(3) |
| ページ | 876-85 |
| 公開日 | 2015-3-1 |
| DOI | 10.1002/eji.201444969 |
| PMID | 25504587 |
| MeSH | Animals Eosinophil Major Basic Protein / genetics Eosinophil Major Basic Protein / immunology Eosinophils / cytology Eosinophils / immunology* Gene Expression Regulation / immunology* Immunity, Innate / physiology* Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / immunology Interleukin-33 Interleukin-5 / genetics Interleukin-5 / immunology Interleukins / genetics Interleukins / immunology* Intra-Abdominal Fat / cytology Intra-Abdominal Fat / immunology* Mice Mice, Knockout |
| IF | 4.404 |
| 引用数 | 27 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | B6N BAC Mouse (RDB07573) B6Ng01–038M13 |