RRC ID 32636
Author Hashiguchi M, Kashiwakura Y, Kojima H, Kobayashi A, Kanno Y, Kobata T.
Title IL-33 activates eosinophils of visceral adipose tissue both directly and via innate lymphoid cells.
Journal Eur J Immunol
Abstract Eosinophils are multifunctional leukocytes involved in allergic reactions as well as adipose tissue regulation. IL-5 is required for eosinophil survival; however, the in vivo mechanisms of eosinophil regulation are not fully understood. A tg mouse model with il5 promoter-driven EGFP expression was established for detecting the IL-5-producing cells in vivo. Il5-egfp tg mice expressed high levels of EGFP in gonadal adipose tissue (GAT) cells. EGFP(+) cells in GAT were mainly group 2 innate lymphoid cells (ILCs). IL-33 preferentially expanded EGFP(+) cells and eosinophils in GAT in vivo. EGFP(+) ILCs were found to upregulate prg2 mRNA expression in GAT eosinophils. These results demonstrate that ILCs activate eosinophils in GAT. The blockage of IL-33Rα, on the other hand, did not impair EGFP(+) ILC numbers but did impair eosinophil numbers in vivo. GAT eosinophils expressed IL-33Rα and IL-33 expanded eosinophil numbers in CD90(+) cell-depleted mice. IL-33 was further observed to induce the expression of retnla and epx mRNA in eosinophils. These findings demonstrate that IL-33 directly activates eosinophils in GAT, and together with our other findings described above, our findings show that IL-33 has dual pathways via which it activates eosinophils in vivo: a direct activation pathway and a group 2 ILC-mediated pathway.
Volume 45(3)
Pages 876-85
Published 2015-3-1
DOI 10.1002/eji.201444969
PMID 25504587
MeSH Animals Eosinophil Major Basic Protein / genetics Eosinophil Major Basic Protein / immunology Eosinophils / cytology Eosinophils / immunology* Gene Expression Regulation / immunology* Immunity, Innate / physiology* Intercellular Signaling Peptides and Proteins / genetics Intercellular Signaling Peptides and Proteins / immunology Interleukin-33 Interleukin-5 / genetics Interleukin-5 / immunology Interleukins / genetics Interleukins / immunology* Intra-Abdominal Fat / cytology Intra-Abdominal Fat / immunology* Mice Mice, Knockout
IF 4.404
Times Cited 27
DNA material B6N BAC Mouse (RDB07573) B6Ng01–038M13