RRC ID 32666
Author Yang HC, Mikami Y, Yazawa K, Taguchi H, Nishimura K, Miyaji M, Branchini ML, Aoki FH, Yamamoto K.
Title Colorimetric MTT assessment of antifungal activity of D0870 against fluconazole-resistant Candida albicans.
Journal Mycoses
Abstract The in vitro antifungal activity of D0870 against eight isolates of fluconazole-resistant Candida albicans was compared with that of itraconazole, ketoconazole and miconazole. The colorimetric MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay was used to assess the antifungal activities. The 50% minimum inhibitory concentration (MIC50) of D0870 was below 0.031 microgram ml-1 for seven isolates and 0.25 microgram ml-1 for one isolate. The activity of D0870 was superior to that of the other azoles. Ketoconazole was the most effective azole next to D0870. Therefore, the new bis-triazole, D0870, is expected to be promising for the therapy of fluconazole-resistant candidosis. The present data also confirmed that the MTT assay may be useful for evaluation of resistance and detection of resistant C. albicans.
Volume 41(11-12)
Pages 477-80
Published 1998-12-1
DOI 10.1111/j.1439-0507.1998.tb00709.x
PMID 9919890
MeSH Antifungal Agents / pharmacology* Candida albicans / drug effects* Candidiasis / drug therapy Candidiasis / microbiology Colorimetry / methods* Drug Resistance, Microbial Fluconazole / pharmacology* Humans Microbial Sensitivity Tests Tetrazolium Salts Thiazoles Triazoles / pharmacology*
IF 3.575
Times Cited 5
Pathogenic microorganisms IFM 40214