| RRC ID |
32666
|
| 著者 |
Yang HC, Mikami Y, Yazawa K, Taguchi H, Nishimura K, Miyaji M, Branchini ML, Aoki FH, Yamamoto K.
|
| タイトル |
Colorimetric MTT assessment of antifungal activity of D0870 against fluconazole-resistant Candida albicans.
|
| ジャーナル |
Mycoses
|
| Abstract |
The in vitro antifungal activity of D0870 against eight isolates of fluconazole-resistant Candida albicans was compared with that of itraconazole, ketoconazole and miconazole. The colorimetric MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay was used to assess the antifungal activities. The 50% minimum inhibitory concentration (MIC50) of D0870 was below 0.031 microgram ml-1 for seven isolates and 0.25 microgram ml-1 for one isolate. The activity of D0870 was superior to that of the other azoles. Ketoconazole was the most effective azole next to D0870. Therefore, the new bis-triazole, D0870, is expected to be promising for the therapy of fluconazole-resistant candidosis. The present data also confirmed that the MTT assay may be useful for evaluation of resistance and detection of resistant C. albicans.
|
| 巻・号 |
41(11-12)
|
| ページ |
477-80
|
| 公開日 |
1998-12-1
|
| DOI |
10.1111/j.1439-0507.1998.tb00709.x
|
| PMID |
9919890
|
| MeSH |
Antifungal Agents / pharmacology*
Candida albicans / drug effects*
Candidiasis / drug therapy
Candidiasis / microbiology
Colorimetry / methods*
Drug Resistance, Microbial
Fluconazole / pharmacology*
Humans
Microbial Sensitivity Tests
Tetrazolium Salts
Thiazoles
Triazoles / pharmacology*
|
| IF |
3.575
|
| 引用数 |
5
|
|
WOS 分野
|
MYCOLOGY
DERMATOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 40214 |
