| RRC ID |
3283
|
| Author |
Maeda S, Ito M, Ando T, Ishimoto Y, Fujisawa Y, Takahashi H, Matsuda A, Sawamura A, Kato S.
|
| Title |
Horizontal transfer of nonconjugative plasmids in a colony biofilm of Escherichia coli.
|
| Journal |
FEMS Microbiol Lett
|
| Abstract |
We tested the possibility of nonconjugative lateral DNA transfer in a colony biofilm of mixed Escherichia coli strains. By simply coculturing a plasmid-free F(-) strain and another F(-) strain harboring a nonconjugative plasmid in a colony biofilm on antibiotic-free agar media, transformed cells were produced within 24-48 h at the frequency of 10(-10)-10(-9) per recipient cell. PCR analysis of the transformed cells demonstrated the occurrence of lateral plasmid transfer. These cells survived until at least day 7 under antibiotic-free conditions. Liquid cultures of the same strains in Luria-Bertani broth produced no or few transformants, suggesting the importance of colony-biofilm formation for plasmid transfer. This is a novel line of evidence indicating that nonconjugative, nonviral horizontal gene transfer can occur between E. coli cells.
|
| Volume |
255(1)
|
| Pages |
115-20
|
| Published |
2006-2-1
|
| DOI |
10.1111/j.1574-6968.2005.00072.x
|
| PII |
FML072
|
| PMID |
16436070
|
| MeSH |
Biofilms / growth & development*
Culture Media
Escherichia coli / genetics*
Escherichia coli / growth & development
Gene Transfer, Horizontal*
Plasmids / genetics*
Transformation, Bacterial / genetics*
|
| IF |
1.987
|
| Times Cited |
43
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Prokaryotes E. coli |
ME8788(CAG18439)
ME8266
ME8584(KF1225)
pHSG299
pGBM1 |
