RRC ID 3287
著者 Nagahama H, Sakamoto Y, Matsumoto K, Hara H.
タイトル RcsA-dependent and -independent growth defects caused by the activated Rcs phosphorelay system in the Escherichia coli pgsA null mutant.
ジャーナル J Gen Appl Microbiol
Abstract In the Escherichia coli pgsA null mutant, which lacks the major acidic phospholipids, the Rcs phosphorelay signal transduction system is activated, causing thermosensitive growth. The mutant grows poorly at 37 degrees C and lyses at 42 degrees C. We showed that the poor growth at 37 degrees C was corrected by disruption of the rcsA gene, which codes for a coregulator protein that interacts with the RcsB response regulator of the phosphorelay system. However, mutant cells still lysed when incubated at 42 degrees C even in the absence of RcsA. We conclude that the activated Rcs phosphorelay in the pgsA null mutant has both RcsA-dependent and -independent growth inhibitory effects. Since the Rcs system has been shown to positively regulate the essential cell division genes ftsA and ftsZ independently of RcsA, we measured cellular levels of the FtsZ protein, but found that the growth defect of the mutant at 42 degrees C did not involve a change in the level of this protein.
巻・号 52(2)
ページ 91-8
公開日 2006-4-1
DOI 10.2323/jgam.52.91
PII JST.JSTAGE/jgam/52.91
PMID 16778352
MeSH Escherichia coli / physiology* Escherichia coli Proteins / physiology* Phosphorylation Signal Transduction / physiology Transferases (Other Substituted Phosphate Groups) / physiology*
IF 1.442
引用数 17
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME8454(SG20043) ME9789