| RRC ID |
3290
|
| 著者 |
Ohniwa RL, Morikawa K, Kim J, Ohta T, Ishihama A, Wada C, Takeyasu K.
|
| タイトル |
Dynamic state of DNA topology is essential for genome condensation in bacteria.
|
| ジャーナル |
EMBO J
|
| Abstract |
In bacteria, Dps is one of the critical proteins to build up a condensed nucleoid in response to the environmental stresses. In this study, we found that the expression of Dps and the nucleoid condensation was not simply correlated in Escherichia coli, and that Fis, which is an E. coli (gamma-Proteobacteria)-specific nucleoid protein, interfered with the Dps-dependent nucleoid condensation. Atomic force microscopy and Northern blot analyses indicated that the inhibitory effect of Fis was due to the repression of the expression of Topoismerase I (Topo I) and DNA gyrase. In the Deltafis strain, both topA and gyrA/B genes were found to be upregulated. Overexpression of Topo I and DNA gyrase enhanced the nucleoid condensation in the presence of Dps. DNA-topology assays using the cell extract showed that the extracts from the Deltafis and Topo I-/DNA gyrase-overexpressing strains, but not the wild-type extract, shifted the population toward relaxed forms. These results indicate that the topology of DNA is dynamically transmutable and that the topology control is important for Dps-induced nucleoid condensation.
|
| 巻・号 |
25(23)
|
| ページ |
5591-602
|
| 公開日 |
2006-11-29
|
| DOI |
10.1038/sj.emboj.7601414
|
| PII |
7601414
|
| PMID |
17093499
|
| PMC |
PMC1679767
|
| MeSH |
Bacterial Outer Membrane Proteins / antagonists & inhibitors
Bacterial Outer Membrane Proteins / genetics
Bacterial Outer Membrane Proteins / metabolism*
Blotting, Northern
Chromosomes, Bacterial / chemistry
Chromosomes, Bacterial / metabolism
Chromosomes, Bacterial / ultrastructure*
DNA Gyrase / genetics
DNA Gyrase / metabolism
DNA Topoisomerases, Type I / genetics
DNA Topoisomerases, Type I / metabolism
DNA, Bacterial / chemistry*
DNA, Bacterial / metabolism
DNA, Bacterial / ultrastructure
Escherichia coli / genetics*
Escherichia coli / metabolism
Escherichia coli Proteins / antagonists & inhibitors
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Escherichia coli Proteins / physiology*
Factor For Inversion Stimulation Protein
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Genome, Bacterial
Microscopy, Atomic Force
Nucleic Acid Conformation*
Oxidative Stress
Transcription Factors / genetics
Transcription Factors / physiology*
|
| IF |
9.889
|
| 引用数 |
61
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 原核生物(大腸菌) |
Keio collection |
