RRC ID 33041
著者 Sakaidani Y, Nomura T, Matsuura A, Ito M, Suzuki E, Murakami K, Nadano D, Matsuda T, Furukawa K, Okajima T.
タイトル O-linked-N-acetylglucosamine on extracellular protein domains mediates epithelial cell-matrix interactions.
ジャーナル Nat Commun
Abstract The O-linked-N-acetylglucosamine (O-GlcNAc) modification of cytoplasmic and nuclear proteins regulates basic cellular functions and is involved in the aetiology of diabetes and neurodegeneration. This intracellular O-GlcNAcylation is catalyzed by a single O-GlcNAc transferase, OGT. Here we report a novel OGT, EOGT, responsible for extracellular O-GlcNAcylation. Although both OGT and EOGT are regulated by hexosamine flux, EOGT localizes to the lumen of the endoplasmic reticulum and transfers GlcNAc to epidermal growth factor-like domains in an OGT-independent manner. Loss of Eogt gives phenotypes similar to those caused by defects in the apical extracellular matrix. Dumpy (Dp), a membrane-anchored extracellular protein, is O-GlcNAcylated, and EOGT is required for Dp-dependent epithelial cell-matrix interactions. Thus, O-GlcNAcylation of secreted and membrane glycoproteins is a novel mediator of cell-cell or cell-matrix interactions at the cell surface.
巻・号 2
ページ 583
公開日 2011-12-13
DOI 10.1038/ncomms1591
PII ncomms1591
PMID 22158438
MeSH Acetylglucosamine / metabolism* Acylation Amino Acid Sequence Animals Cell Communication / genetics* Drosophila Proteins / chemistry Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / enzymology Drosophila melanogaster / genetics* Epidermal Growth Factor / chemistry Epidermal Growth Factor / genetics Epidermal Growth Factor / metabolism Epithelial Cells / cytology Epithelial Cells / metabolism* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism* Hexosamines / metabolism Mice Molecular Sequence Data N-Acetylglucosaminyltransferases / chemistry N-Acetylglucosaminyltransferases / genetics N-Acetylglucosaminyltransferases / metabolism* Plasmids Protein Binding Protein Processing, Post-Translational / genetics Protein Structure, Tertiary RNA Interference Sequence Homology, Amino Acid Signal Transduction Transfection
IF 12.121
引用数 104
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ 9867R-1 9867R-3 15637R-1