RRC ID 3305
Author Cui T, Moro-oka N, Ohsumi K, Kodama K, Ohshima T, Ogasawara N, Mori H, Wanner B, Niki H, Horiuchi T.
Title Escherichia coli with a linear genome.
Journal EMBO Rep
Abstract Chromosomes in eukaryotes are linear, whereas those of most, but not all, prokaryotes are circular. To explore the effects of possessing a linear genome on prokaryotic cells, we linearized the Escherichia coli genome using the lysogenic lambda-like phage N15. Linear genome E. coli were viable and their genome structure was stable. There were no appreciable differences between cells with linear or circular genomes in growth rates, cell and nucleoid morphologies, genome-wide gene expression (with a few exceptions), and DNA gyrase- and topoisomerase IV-dependent growth. However, under dif-defective conditions, only cells with a circular genome developed an abnormal phenotype. Microscopy indicated that the ends of the linear genome, but not the circular genome, were separated and located at each end of a new-born cell. When tos - the cis-element required for linearization - was inserted into different chromosomal sites, those strains with the genome termini that were more remote from dif showed greater growth deficiencies.
Volume 8(2)
Pages 181-7
Published 2007-2-1
DOI 10.1038/sj.embor.7400880
PII 7400880
PMID 17218953
PMC PMC1796773
MeSH Cell Proliferation Chromosomes, Bacterial / chemistry* Coliphages / metabolism Escherichia coli / cytology* Escherichia coli / genetics* Gene Expression Regulation, Bacterial / genetics Genome, Bacterial / genetics* Microarray Analysis
IF 8.383
Times Cited 44
Prokaryotes E. coli pBAD24 ME9028 ME9273