論文 - 詳細
| RRC ID | 3309 |
|---|---|
| 著者 | Fuhrer T, Chen L, Sauer U, Vitkup D. |
| タイトル | Computational prediction and experimental verification of the gene encoding the NAD+/NADP+-dependent succinate semialdehyde dehydrogenase in Escherichia coli. |
| ジャーナル | J Bacteriol |
| Abstract |
Although NAD(+)-dependent succinate semialdehyde dehydrogenase activity was first described in Escherichia coli more than 25 years ago, the responsible gene has remained elusive so far. As an experimental proof of concept for a gap-filling algorithm for metabolic networks developed earlier, we demonstrate here that the E. coli gene yneI is responsible for this activity. Our biochemical results demonstrate that the yneI-encoded succinate semialdehyde dehydrogenase can use either NAD(+) or NADP(+) to oxidize succinate semialdehyde to succinate. The gene is induced by succinate semialdehyde, and expression data indicate that yneI plays a unique physiological role in the general nitrogen metabolism of E. coli. In particular, we demonstrate using mutant growth experiments that the yneI gene has an important, but not essential, role during growth on arginine and probably has an essential function during growth on putrescine as the nitrogen source. The NADP(+)-dependent succinate semialdehyde dehydrogenase activity encoded by the functional homolog gabD appears to be important for nitrogen metabolism under N limitation conditions. The yneI-encoded activity, in contrast, functions primarily as a valve to prevent toxic accumulation of succinate semialdehyde. Analysis of available genome sequences demonstrated that orthologs of both yneI and gabD are broadly distributed across phylogenetic space. |
| 巻・号 | 189(22) |
| ページ | 8073-8 |
| 公開日 | 2007-11-1 |
| DOI | 10.1128/JB.01027-07 |
| PII | JB.01027-07 |
| PMID | 17873044 |
| PMC | PMC2168661 |
| MeSH | Computer Simulation* Escherichia coli / enzymology* Escherichia coli / genetics Gene Deletion Gene Expression Regulation, Bacterial Genes, Bacterial Succinate-Semialdehyde Dehydrogenase / genetics* Succinate-Semialdehyde Dehydrogenase / metabolism |
| IF | 3.006 |
| 引用数 | 44 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 36 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062 (BW25113) |