Reference - Detail
| RRC ID | 33117 |
|---|---|
| Author | Kimoto M, Tsubota T, Uchino K, Sezutsu H, Takiya S. |
| Title | LIM-homeodomain transcription factor Awh is a key component activating all three fibroin genes, fibH, fibL and fhx, in the silk gland of the silkworm, Bombyx mori. |
| Journal | Insect Biochem Mol Biol |
| Abstract |
In the silkworm Bombyx mori, three fibroin genes, fibroin-heavy-chain (fibH), fibroin-light-chain (fibL) and fibrohexamerin (fhx), are coexpressed only in the posterior silk gland (PSG) cells, while the sericin genes encoding silk glue proteins are expressed in the middle silk gland (MSG) cells. Silk gland factor-2 (SGF-2) is a PSG-specific activator complex of fibH, composed of a LIM-homeodomain protein, Awh, and its cofactors, Ldb and Lcaf. We investigated whether SGF-2 can activate other fibroin genes using transgenic silkworms. The genes for Ldb and Lcaf were expressed ubiquitously in various tissues, while the gene for Awh was expressed strictly specific in PSG of the wild type silkworms. Misexpression of Awh in transgenic silkworms induced ectopic expression of fibL and fhx as well as fibH in MSG. Coincidently with the induction of fibL and fhx by Awh, binding of SGF-2 to the promoter of fibL and fhx was detected in vitro, and SGF-2 binds directly to the fhx core promoter. Ectopic expression of the fibroin genes was observed at high levels in the middle part of MSG. Moreover, fibL and fhx were induced in the anterior silk gland (ASG) of the transgenic silkworms, but fibH was not. These results indicate that Awh is a key activator of all three fibroin genes, and the activity is probably regulated in conjunction with additional factors. |
| Volume | 56 |
| Pages | 29-35 |
| Published | 2015-1-1 |
| DOI | 10.1016/j.ibmb.2014.11.003 |
| PII | S0965-1748(14)00177-5 |
| PMID | 25449130 |
| MeSH | Animals Animals, Genetically Modified Base Sequence Bombyx / genetics Bombyx / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Fibroins / biosynthesis Fibroins / genetics* Glycoproteins / biosynthesis Glycoproteins / genetics Homeodomain Proteins In Situ Hybridization LIM-Homeodomain Proteins / genetics LIM-Homeodomain Proteins / metabolism* Larva Molecular Sequence Data Promoter Regions, Genetic Real-Time Polymerase Chain Reaction Sericins / metabolism Silk / metabolism Transcription Factors Transcription, Genetic / physiology* |
| IF | 3.827 |
| Times Cited | 15 |
| WOS Category | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Silkworms | |