| RRC ID |
33122
|
| 著者 |
Kokusho R, Kawamoto M, Koyano Y, Sugano S, Suzuki Y, Shimada T, Katsuma S.
|
| タイトル |
Bombyx mori nucleopolyhedrovirus actin rearrangement-inducing factor 1 enhances systemic infection in B. mori larvae.
|
| ジャーナル |
J Gen Virol
|
| Abstract |
The actin rearrangement-inducing factor 1 (arif-1) gene is a baculoviral early gene conserved in most alphabaculoviruses. Previous studies reported that Autographa californica nucleopolyhedrovirus ARIF-1 protein induces filamentous actin concentration on the plasma membrane during the early stage of infection in Trichoplusia ni TN-368 cells, but its role in larval infection remains unknown. In this study, we performed behavioural screening using Bombyx mori larvae infected with Bombyx mori nucleopolyhedrovirus (BmNPV) mutants and found that larvae infected with arif-1-mutated BmNPVs did not show locomotor hyperactivity that was normally observed in BmNPV-infected larvae. arif-1-deficient BmNPVs also showed reduced pathogenicity and total viral propagation in B. mori larvae, whereas viral propagation of arif-1-deficient viruses was comparable with that of control viruses in B. mori cultured cells. An arif-1-defective BmNPV expressing the GFP gene (gfp) was used to monitor the progression of infection in B. mori larvae. GFP expression and quantitative reverse transcription-PCR analyses revealed that infection by the arif-1-disrupted virus was significantly delayed in trachea, fat body, suboesophageal ganglion and brain. These results indicated that BmNPV ARIF-1 enhanced systemic infection in B. mori larvae.
|
| 巻・号 |
96(Pt 7)
|
| ページ |
1938-46
|
| 公開日 |
2015-7-1
|
| DOI |
10.1099/vir.0.000130
|
| PII |
vir.0.000130
|
| PMID |
25809914
|
| MeSH |
Actins / metabolism*
Animals
Bombyx / virology*
Gene Knockout Techniques
Host-Pathogen Interactions*
Larva / virology
Locomotion
Membrane Proteins / genetics
Membrane Proteins / metabolism
Nucleopolyhedroviruses / genetics
Nucleopolyhedroviruses / growth & development
Nucleopolyhedroviruses / physiology*
Phosphoproteins / genetics
Phosphoproteins / metabolism
Protein Multimerization*
Viral Proteins / genetics
Viral Proteins / metabolism*
Virulence
|
| IF |
3.376
|
| 引用数 |
3
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
VIROLOGY
|
| リソース情報 |
| カイコ |
|
