RRC ID 33128
著者 Matsumoto H, Ueno C, Nakamura Y, Kinjoh T, Ito Y, Shimura S, Noda H, Imanishi S, Mita K, Fujiwara H, Hiruma K, Shinoda T, Kamimura M.
タイトル Identification of two juvenile hormone inducible transcription factors from the silkworm, Bombyx mori.
ジャーナル J Insect Physiol
Abstract Juvenile hormone (JH) regulates many physiological processes in insects. However, the signal cascades in which JH is active have not yet been fully elucidated, particularly in comparison to another major hormone ecdysteroid. Here we identified two JH inducible transcription factors as candidate components of JH signaling pathways in the silkworm, Bombyx mori. DNA microarray analysis showed that expression of two transcription factor genes, E75 and Enhancer of split mβ (E(spl)mβ), was induced by juvenile hormone I (JH I) in NIAS-Bm-aff3 cells. Real time RT-PCR analysis confirmed that expression of four E75 isoforms (E75A, E75B, E75C and E75D) and E(spl)mβ was 3-8 times greater after JH I addition. Addition of the protein synthesis inhibitor cycloheximide did not suppress JH-induced expression of the genes, indicating that they were directly induced by JH. JH-induced expression of E75 and E(spl)mβ was also observed in four other B. mori cell lines and in larval hemocytes of final instar larvae. Notably, E75A expression was induced very strongly in larval hemocytes by topical application of the JH analog fenoxycarb; the level of induced expression was comparable to that produced by feeding larvae with 20-hydroxyecdysone. These results suggest that E75 and E(spl)mβ are general and direct target genes of JH and that the transcription factors encoded by these genes play important roles in JH signaling.
巻・号 80
ページ 31-41
公開日 2015-9-1
DOI 10.1016/j.jinsphys.2015.02.011
PII S0022-1910(15)00046-3
PMID 25770979
MeSH Amino Acid Sequence Animals Bombyx / chemistry Bombyx / genetics* Bombyx / growth & development Bombyx / metabolism Ecdysteroids / biosynthesis Ecdysterone / biosynthesis Gene Expression Regulation, Developmental Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / metabolism Juvenile Hormones / metabolism* Larva / chemistry Larva / genetics Larva / growth & development Larva / metabolism Molecular Sequence Data Sequence Alignment Transcription Factors / chemistry Transcription Factors / genetics* Transcription Factors / metabolism Up-Regulation*
IF 2.246
引用数 2
WOS 分野 ENTOMOLOGY PHYSIOLOGY ZOOLOGY
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