RRC ID 33217
Author Matsuda T, Cepko CL.
Title Controlled expression of transgenes introduced by in vivo electroporation.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract In vivo electroporation is a powerful technique for the introduction of genes into organisms. Temporal and spatial regulation of expression of introduced genes, or of RNAi, would further enhance the utility of this method. Here we demonstrate conditional regulation of gene expression from electroporated plasmids in the postnatal rat retina and the embryonic mouse brain. For temporal regulation, Cre/loxP-mediated inducible expression vectors were used in combination with a vector expressing a conditionally active form of Cre recombinase, which is activated by 4-hydroxytamoxifen. Onset of gene expression was regulated by the timing of 4-hydroxytamoxifen administration. For spatial regulation, transgenes were expressed by using promoters specific for rod photoreceptors, bipolar cells, amacrine cells, Müller glia or progenitor cells. Combinations of these constructs will facilitate a variety of experiments, including cell-type-specific gene misexpression, conditional RNAi, and fate mapping of progenitor and precursor cells.
Volume 104(3)
Pages 1027-32
Published 2007-1-16
DOI 10.1073/pnas.0610155104
PII 0610155104
PMID 17209010
PMC PMC1764220
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Cell Line Cell Lineage Electroporation / methods* Gene Expression / genetics* Gene Expression Regulation Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Humans Integrases / genetics Integrases / metabolism Promoter Regions, Genetic / genetics RNA Interference Rats Retina / cytology Retina / metabolism Transcription Factor HES-1 Transfection / methods*
IF 9.504
Times Cited 250
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material pCALNL5 (RDB01862) pxCANCre (RDB01675).