論文 - 詳細
| RRC ID | 3323 |
|---|---|
| 著者 | Maeda T, Sanchez-Torres V, Wood TK. |
| タイトル | Escherichia coli hydrogenase 3 is a reversible enzyme possessing hydrogen uptake and synthesis activities. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
In the past, it has been difficult to discriminate between hydrogen synthesis and uptake for the three active hydrogenases in Escherichia coli (hydrogenase 1, 2, and 3); however, by combining isogenic deletion mutations from the Keio collection, we were able to see the role of hydrogenase 3. In a cell that lacks hydrogen uptake via hydrogenase 1 (hyaB) and via hydrogenase 2 (hybC), inactivation of hydrogenase 3 (hycE) decreased hydrogen uptake. Similarly, inactivation of the formate hydrogen lyase complex, which produces hydrogen from formate (fhlA) in the hyaB hybC background, also decreased hydrogen uptake; hence, hydrogenase 3 has significant hydrogen uptake activity. Moreover, hydrogen uptake could be restored in the hyaB hybC hycE and hyaB hybC fhlA mutants by expressing hycE and fhlA, respectively, from a plasmid. The hydrogen uptake results were corroborated using two independent methods (both filter plate assays and a gas-chromatography-based hydrogen uptake assay). A 30-fold increase in the forward reaction, hydrogen formation by hydrogenase 3, was also detected for the strain containing active hydrogenase 3 activity but no hydrogenase 1 or 2 activity relative to the strain lacking all three hydrogenases. These results indicate clearly that hydrogenase 3 is a reversible hydrogenase. |
| 巻・号 | 76(5) |
| ページ | 1035-42 |
| 公開日 | 2007-10-1 |
| DOI | 10.1007/s00253-007-1086-6 |
| PMID | 17668201 |
| MeSH | Chromatography, Gas Culture Media Escherichia coli / enzymology* Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli Proteins / metabolism Fructose / metabolism Hydrogen / metabolism* Hydrogenase / genetics Hydrogenase / metabolism* Oxidation-Reduction Paraquat / metabolism |
| IF | 3.53 |
| 引用数 | 69 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW2701 JW2472 JW0955 JW2962 JW2691 |