RRC ID 3324
著者 Maeda T, Sanchez-Torres V, Wood TK.
タイトル Enhanced hydrogen production from glucose by metabolically engineered Escherichia coli.
ジャーナル Appl Microbiol Biotechnol
Abstract To utilize fermentative bacteria for producing the alternative fuel hydrogen, we performed successive rounds of P1 transduction from the Keio Escherichia coli K-12 library to introduce multiple, stable mutations into a single bacterium to direct the metabolic flux toward hydrogen production. E. coli cells convert glucose to various organic acids (such as succinate, pyruvate, lactate, formate, and acetate) to synthesize energy and hydrogen from formate by the formate hydrogen-lyase (FHL) system that consists of hydrogenase 3 and formate dehydrogenase-H. We altered the regulation of FHL by inactivating the repressor encoded by hycA and by overexpressing the activator encoded by fhlA, removed hydrogen uptake activity by deleting hyaB (hydrogenase 1) and hybC (hydrogenase 2), redirected glucose metabolism to formate by using the fdnG, fdoG, narG, focA, focB, poxB, and aceE mutations, and inactivated the succinate and lactate synthesis pathways by deleting frdC and ldhA, respectively. The best of the metabolically engineered strains, BW25113 hyaB hybC hycA fdoG frdC ldhA aceE, increased hydrogen production 4.6-fold from glucose and increased the hydrogen yield twofold from 0.65 to 1.3 mol H(2)/mol glucose (maximum, 2 mol H(2)/mol glucose).
巻・号 77(4)
ページ 879-90
公開日 2007-12-1
DOI 10.1007/s00253-007-1217-0
PMID 17938909
MeSH Escherichia coli K12 / enzymology Escherichia coli K12 / genetics* Escherichia coli Proteins Formate Dehydrogenases / genetics* Formate Dehydrogenases / metabolism Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic* Genes, Bacterial* Genetic Enhancement* Hydrogen / metabolism* Hydrogenase / genetics* Hydrogenase / metabolism Multienzyme Complexes / genetics* Multienzyme Complexes / metabolism
IF 3.53
引用数 108
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
リソース情報
原核生物(大腸菌) ME9062(BW25113) Keio ASKA