Reference - Detail
| RRC ID | 3327 |
|---|---|
| Author | Miyakawa T, Sawano Y, Miyazono K, Hatano K, Tanokura M. |
| Title | Crystallization and preliminary X-ray analysis of ginkbilobin-2 from Ginkgo biloba seeds: a novel antifungal protein with homology to the extracellular domain of plant cysteine-rich receptor-like kinases. |
| Journal | Acta Crystallogr Sect F Struct Biol Cryst Commun |
| Abstract |
The antifungal protein ginkbilobin-2 (Gnk2) from Ginkgo biloba seeds does not show homology to other pathogenesis-related proteins, but does show homology to the extracellular domain of plant cysteine-rich receptor-like kinases. Native Gnk2 purified from ginkgo nuts and the selenomethionine derivative of recombinant Gnk2 (SeMet-rGnk2) were crystallized by the sitting-drop vapour-diffusion method using different precipitants. X-ray diffraction data were collected from Gnk2 at 2.38 A resolution and from SeMet-rGnk2 at 2.79 A resolution using a synchrotron-radiation source. The crystals of both proteins belonged to the primitive cubic space group P2(1)3, with unit-cell parameters a = b = c = 143.2 A. |
| Volume | 63(Pt 9) |
| Pages | 737-9 |
| Published | 2007-9-1 |
| DOI | 10.1107/S1744309107034793 |
| PII | S1744309107034793 |
| PMID | 17768341 |
| PMC | PMC2376327 |
| MeSH | Antimicrobial Cationic Peptides / chemistry* Antimicrobial Cationic Peptides / isolation & purification Crystallization Cysteine Ginkgo biloba Plant Proteins / chemistry Plant Proteins / isolation & purification Plants / enzymology Protein Kinases / chemistry Protein Kinases / isolation & purification Seeds / chemistry* X-Ray Diffraction |
| IF | 0.968 |
| Times Cited | 9 |
| WOS Category | CRYSTALLOGRAPHY BIOPHYSICS BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Prokaryotes E. coli | ME7982 (JM109 pDsbCD1) |