論文 - 詳細
| RRC ID | 3333 |
|---|---|
| 著者 | Reddy M. |
| タイトル | Role of FtsEX in cell division of Escherichia coli: viability of ftsEX mutants is dependent on functional SufI or high osmotic strength. |
| ジャーナル | J Bacteriol |
| Abstract |
In Escherichia coli, at least 12 proteins, FtsZ, ZipA, FtsA, FtsE/X, FtsK, FtsQ, FtsL, FtsB, FtsW, FtsI, FtsN, and AmiC, are known to localize to the septal ring in an interdependent and sequential pathway to coordinate the septum formation at the midcell. The FtsEX complex is the latest recruit of this pathway, and unlike other division proteins, it is shown to be essential only on low-salt media. In this study, it is shown that ftsEX null mutations are not only salt remedial but also osmoremedial, which suggests that FtsEX may not be involved in salt transport as previously thought. Increased coexpression of cell division proteins FtsQ-FtsA-FtsZ or FtsN alone restored the growth defects of ftsEX mutants. ftsEX deletion exacerbated the defects of most of the mutants affected in Z ring localization and septal assembly; however, the ftsZ84 allele was a weak suppressor of ftsEX. The viability of ftsEX mutants in high-osmolarity conditions was shown to be dependent on the presence of a periplasmic protein, SufI, a substrate of twin-arginine translocase. In addition, SufI in multiple copies could substitute for the functions of FtsEX. Taken together, these results suggest that FtsE and FtsX are absolutely required for the process of cell division in conditions of low osmotic strength for the stability of the septal ring assembly and that, during high-osmolarity conditions, the FtsEX and SufI functions are redundant for this essential process. |
| 巻・号 | 189(1) |
| ページ | 98-108 |
| 公開日 | 2007-1-1 |
| DOI | 10.1128/JB.01347-06 |
| PII | JB.01347-06 |
| PMID | 17071757 |
| PMC | PMC1797223 |
| MeSH | ATP-Binding Cassette Transporters / physiology* Cell Cycle Proteins / physiology* Cell Division Cystic Fibrosis Transmembrane Conductance Regulator / physiology* Escherichia coli / cytology* Escherichia coli Proteins / physiology* Osmolar Concentration |
| IF | 3.006 |
| 引用数 | 66 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pMA2 |