論文 - 詳細
| RRC ID | 33348 |
|---|---|
| 著者 | Barriault D, Sylvestre M. |
| タイトル | A ColE1-compatible expression vector for the production of His-tagged fusion proteins. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
Plasmid pDB31 is a ColE1-compatible expression vector based on the p15A origin of replication. It is designed to express His-tagged fusion proteins in cells co-hosting a compatible expression vector. It was constructed by assembling the operator/promoter region plus the 6xHis and the multiple cloning site of pQE31 (QIAGEN) with the p15A origin of replication plus KanR of pGP1-2. The plasmid was found to be stable in Escherichia coli strains BL21 and DH11S. It was used to produce and purify the ferredoxin reductase component of Comamonas testosteroni B-356 biphenyl dioxygenase inside a clone hosting the remaining dioxygenase genes on a compatible plasmid. |
| 巻・号 | 75(4) |
| ページ | 293-7 |
| 公開日 | 1999-5-1 |
| DOI | 10.1023/a:1002070630330 |
| PMID | 10510716 |
| MeSH | DNA, Recombinant / genetics Escherichia coli / enzymology* Escherichia coli / genetics Ferredoxin-NADP Reductase / biosynthesis* Ferredoxin-NADP Reductase / genetics Genetic Vectors / chemical synthesis* Genetic Vectors / genetics Gram-Negative Aerobic Rods and Cocci / enzymology Histidine / genetics* Iron-Sulfur Proteins* Oxygenases / biosynthesis* Oxygenases / genetics Recombinant Fusion Proteins / biosynthesis* Recombinant Fusion Proteins / genetics |
| IF | 2.674 |
| 引用数 | 3 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 原核生物(大腸菌) | pDB31 |