RRC ID 33354
著者 Miyaji T, Kuromori T, Takeuchi Y, Yamaji N, Yokosho K, Shimazawa A, Sugimoto E, Omote H, Ma JF, Shinozaki K, Moriyama Y.
タイトル AtPHT4;4 is a chloroplast-localized ascorbate transporter in Arabidopsis.
ジャーナル Nat Commun
Abstract Ascorbate is an antioxidant and coenzyme for various metabolic reactions in vivo. In plant chloroplasts, high ascorbate levels are required to overcome photoinhibition caused by strong light. However, ascorbate is synthesized in the mitochondria and the molecular mechanisms underlying ascorbate transport into chloroplasts are unknown. Here we show that AtPHT4;4, a member of the phosphate transporter 4 family of Arabidopsis thaliana, functions as an ascorbate transporter. In vitro analysis shows that proteoliposomes containing the purified AtPHT4;4 protein exhibit membrane potential- and Cl(-)-dependent ascorbate uptake. The AtPHT4;4 protein is abundantly expressed in the chloroplast envelope membrane. Knockout of AtPHT4;4 results in decreased levels of the reduced form of ascorbate in the leaves and the heat dissipation process of excessive energy during photosynthesis is compromised. Taken together, these observations indicate that the AtPHT4;4 protein is an ascorbate transporter at the chloroplast envelope membrane, which may be required for tolerance to strong light stress.
巻・号 6
ページ 5928
公開日 2015-1-5
DOI 10.1038/ncomms6928
PII ncomms6928
PMID 25557369
PMC PMC4308718
MeSH Anion Transport Proteins / genetics* Anion Transport Proteins / metabolism* Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Ascorbic Acid / metabolism* Chloroplasts / metabolism* DNA Primers / genetics DNA, Complementary / genetics Fluorescence Gene Knockout Techniques Immunohistochemistry In Vitro Techniques Light Membrane Transport Proteins / genetics* Membrane Transport Proteins / metabolism Polymerase Chain Reaction Stress, Physiological / genetics
IF 12.121
引用数 67
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda05103 pda11419 pda07395 pda12511 pda04259