論文 - 詳細
| RRC ID | 33368 |
|---|---|
| 著者 | De Moerlooze L, Struman I, Renard A, Martial JA. |
| タイトル | Stabilization of T7-promoter-based pARHS expression vectors using the parB locus. |
| ジャーナル | Gene |
| Abstract |
We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid R1 was introduced into the plasmid, pAR3040, to construct the pARHS vectors. These vectors are stable for at least 60 cell generations, even in the absence of selection by an antibiotic present in the culture media, both with or without IPTG induction. |
| 巻・号 | 119(1) |
| ページ | 91-3 |
| 公開日 | 1992-9-21 |
| DOI | 10.1016/0378-1119(92)90070-6 |
| PII | 0378-1119(92)90070-6 |
| PMID | 1398095 |
| MeSH | Base Sequence DNA Gene Expression Regulation / drug effects Genetic Vectors* Humans Isopropyl Thiogalactoside / pharmacology Molecular Sequence Data Plasmids* Prolactin / genetics T-Phages / genetics* |
| IF | 2.984 |
| 引用数 | 13 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 原核生物(大腸菌) | pARHS2 pARHS3 |