| RRC ID |
33385
|
| 著者 |
Schäfer A, Tauch A, Jäger W, Kalinowski J, Thierbach G, Pühler A.
|
| タイトル |
Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum.
|
| ジャーナル |
Gene
|
| Abstract |
Here we describe small mobilizable vectors based on the Escherichia coli plasmids pK18 and pK19. We combined the useful properties of the pK plasmids (e.g., multiple cloning site, lacZ alpha fragment, sequencing with M13 primers) with the broad-host-range transfer machinery of plasmid RP4 and a modified sacB gene from Bacillus subtilis. The new pK derivatives can be transferred by RP4-mediated conjugation into a wide range of Gram- and Gram+ bacteria, and should facilitate gene disruption and allelic exchange by homologous recombination. As an application example, the generation of a defined deletion of the hom-thrB genes in the chromosome of the Gram+ bacterium Corynebacterium glutamicum is presented.
|
| 巻・号 |
145(1)
|
| ページ |
69-73
|
| 公開日 |
1994-7-22
|
| DOI |
10.1016/0378-1119(94)90324-7
|
| PII |
0378-1119(94)90324-7
|
| PMID |
8045426
|
| MeSH |
Chromosomes, Bacterial
Cloning, Molecular
Corynebacterium / genetics*
Escherichia coli / genetics*
Genes, Bacterial
Genetic Vectors*
Plasmids*
Restriction Mapping
Sequence Deletion
|
| IF |
2.984
|
| 引用数 |
1729
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 原核生物(大腸菌) |
pK18mob
pK18mobsacB
pK19mob |
