Reference - Detail
| RRC ID | 33391 |
|---|---|
| Author | Mdluli KE, Treit JD, Kerr VJ, Nano FE. |
| Title | New vectors for the in vitro generation of alkaline phosphatase fusions to proteins encoded by G+C-rich DNA. |
| Journal | Gene |
| Abstract |
Phagemid vectors were constructed to allow fusions of alkaline phosphatase to proteins encoded by G+C-rich DNA, by engineering a BstBI site (TT/CGAA) in front of a phoA gene that lacks an encoded signal peptide. Three vectors (pJDT1, pJDT2 and pJDT3), each with phoA in a different reading frame with respect to the BstBI site, were produced; a lacP region is present in each plasmid upstream of the BstBI site. The presence of the BstBI site allows the random cloning of G+C-rich DNA digested with a number of restriction enzymes that generate cohesive ends. |
| Volume | 155(1) |
| Pages | 133-4 |
| Published | 1995-3-21 |
| DOI | 10.1016/0378-1119(94)00909-c |
| PII | 037811199400909C |
| PMID | 7698658 |
| MeSH | Alkaline Phosphatase / biosynthesis* Alkaline Phosphatase / genetics Amino Acid Sequence Base Sequence DNA, Bacterial / genetics Genetic Vectors* Molecular Sequence Data Mycobacterium tuberculosis / genetics Polymerase Chain Reaction Recombinant Fusion Proteins / biosynthesis* Recombinant Fusion Proteins / genetics |
| IF | 2.984 |
| Times Cited | 12 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | pJDT1 pJDT3 |