| RRC ID |
33402
|
| Author |
Derbyshire KM.
|
| Title |
An IS903-based vector for transposon mutagenesis and the isolation of gene fusions.
|
| Journal |
Gene
|
| Abstract |
A derivative of the IS903 transposon (Tn) is described that is capable of creating lacZ gene fusions upon transposition. It should find wide use as a tool for Tn mutagenesis in bacteria since it can be used both to generate mutants and to examine gene expression. The transposase-encoding gene (tnp) is located outside the Tn in the vector, thus Tn insertions into a genome are stably maintained in the absence of its cis-acting transposase (Tnp). The element carries a KmR gene allowing for the direct selection of transposition events in hosts that cannot support pBR322 plasmid replication and facilitating the subcloning of genes into which the Tn has inserted.
|
| Volume |
165(1)
|
| Pages |
143-4
|
| Published |
1995-11-7
|
| DOI |
10.1016/0378-1119(95)00512-5
|
| PII |
0378111995005125
|
| PMID |
7489906
|
| MeSH |
Cloning, Molecular*
DNA Transposable Elements / genetics*
Genetic Vectors*
Mutagenesis, Insertional*
Plasmids / genetics
|
| IF |
2.984
|
| Times Cited |
8
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Prokaryotes E. coli |
pKD368 |
