論文 - 詳細
| RRC ID | 33409 |
|---|---|
| 著者 | Balbás P, Alexeyev M, Shokolenko I, Bolivar F, Valle F. |
| タイトル | A pBRINT family of plasmids for integration of cloned DNA into the Escherichia coli chromosome. |
| ジャーナル | Gene |
| Abstract |
Plasmid pBRINT is an efficient vector for chromosomal integration of cloned DNA into the lacZ gene of Escherichia coli [Balbás et al., Gene 136(1993) 211-213]. A family of related plasmids containing different antibiotic-resistance markers (CmR or GmR or KmR) and a larger multiple cloning site (MCS) has been constructed. This set of plasmids, whose integration efficiencies are as good as those obtained with the prototype plasmid pBRINT, constitutes a collection of tools that allow rapid and easy integration of cloned DNA, at the chromosomal level. Their functionality as integration vectors has been ascertained by integrating the Vitreoscilla sp. hemoglobin-encoding gene and the Photobacterium leiognathi lux genes. To evaluate the level of expression obtained after chromosomal integration, we constructed strains carrying one or two copies of the cat gene integrated in the chromosome, and compared their enzymatic activities with those obtained from a strain carrying cat on a multicopy plasmid. |
| 巻・号 | 172(1) |
| ページ | 65-9 |
| 公開日 | 1996-6-12 |
| DOI | 10.1016/0378-1119(96)00028-5 |
| PII | 0378-1119(96)00028-5 |
| PMID | 8654993 |
| MeSH | Base Sequence Chloramphenicol O-Acetyltransferase / genetics Chromosomes, Bacterial* Cloning, Molecular DNA / genetics* Escherichia coli / genetics* Molecular Sequence Data Plasmids* Transduction, Genetic |
| IF | 2.984 |
| 引用数 | 18 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pBRINT-Cm pBRINT-Gm pBRINT-Km |