論文 - 詳細
| RRC ID | 33412 |
|---|---|
| 著者 | Snaith MR, Kilby NJ, Murray JA. |
| タイトル | An Escherichia coli system for assay of F1p site-specific recombination on substrate plasmids. |
| ジャーナル | Gene |
| Abstract |
We have developed an Escherichia coli system for testing the behaviour of plasmids carrying target sites for the F1p site-specific recombinase. The E. coli strain BL-FLP is described, which carries a chromosomally integrated bacteriophage T7 RNA polymerase gene expressed from a lac promoter, and harbours the plasmid pMS40.pMS40 has the features: (i) it carries the FLP recombinase gene under the control of a bacteriophage T7 promoter, (ii) it confers kanamycin resistance, and (iii) it uses an R6K origin of replication; these two latter features make it compatible with most conventional cloning vectors. Substrate plasmids carrying F1p-recognition targets (FRT) are transformed into BL-FLP, and the consequences of F1p-mediated recombination can be analysed after subsequent extraction of plasmid DNA. We show that this system is capable of base-perfect F1p-mediated recombination on plasmid substrates. We also present a corrected sequence of the commonly used F1p substrate plasmid, pNEO beta GAL (O'Gorman et al. (1991) Science 251, 1351-1355). |
| 巻・号 | 180(1-2) |
| ページ | 225-7 |
| 公開日 | 1996-11-21 |
| DOI | 10.1016/s0378-1119(96)00449-0 |
| PII | S0378-1119(96)00449-0 |
| PMID | 8973372 |
| MeSH | Binding Sites DNA Nucleotidyltransferases / genetics DNA Nucleotidyltransferases / metabolism* Escherichia coli / genetics* Molecular Sequence Data Plasmids / genetics* Plasmids / metabolism Recombination, Genetic* |
| IF | 2.984 |
| 引用数 | 6 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pMS40 |