Reference - Detail
| RRC ID | 33416 |
|---|---|
| Author | Hennecke H, Günther I, Binder F. |
| Title | A novel cloning vector for the direct selection of recombinant DNA in E. coli. |
| Journal | Gene |
| Abstract |
A new plasmid cloning vector (pHE3) is described carrying dominant p-fluorophenylalanine-sensitivity (pheS) and chloramphenicol-resistance markers. This vector is used in combination with a p-fluorophenylalanine-resistant Escherichia coli recipient (strain RR28). Foreign DNA can be cloned into pHE3 leading to insertional inactivation of pheS. Transformation of RR28, and plating on minimal medium with chloramphenicol plus p-fluorophenylalanine (pfp) directly selects for colonies containing recombinant DNA. |
| Volume | 19(2) |
| Pages | 231-4 |
| Published | 1982-9-1 |
| DOI | 10.1016/0378-1119(82)90011-7 |
| PII | 0378-1119(82)90011-7 |
| PMID | 6293932 |
| MeSH | Cloning, Molecular* DNA Restriction Enzymes DNA, Recombinant / metabolism* Escherichia coli / drug effects Escherichia coli / genetics* Genes, Dominant* Plasmids Transformation, Genetic p-Fluorophenylalanine / pharmacology |
| IF | 2.984 |
| Times Cited | 93 |
| WOS Category | GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | pHE3 |