RRC ID 33417
Author Blondelet-Rouault MH, Weiser J, Lebrihi A, Branny P, Pernodet JL.
Title Antibiotic resistance gene cassettes derived from the omega interposon for use in E. coli and Streptomyces.
Journal Gene
Abstract Three antibiotic resistance gene cassettes, derived from the omega interposon (Prentki and Krisch (1984) Gene 29, 303-313) were constructed. These cassettes carry different antibiotic resistance genes, conferring resistance to geneticin, hygromycin or viomycin, flanked by short inverted repeats containing transcription and translation termination signals and synthetic polylinkers. These cassettes were designated omega aac, omega hyg and omega vph. Resistance phenotypes conferred by these constructions are selectable in E. coli and Streptomyces. These cassettes can be used for insertional mutagenesis or for vector construction.
Volume 190(2)
Pages 315-7
Published 1997-5-6
PII S0378111997000140
PMID 9197550
MeSH DNA Transposable Elements / genetics* Drug Resistance, Microbial / genetics* Escherichia coli / drug effects Escherichia coli / genetics* Molecular Sequence Data Streptomyces / drug effects Streptomyces / genetics*
IF 2.498
Times Cited 164
Prokaryotes E. coli pHP45omega pHP45omegaaac pHP45omegahyg