| RRC ID |
33417
|
| 著者 |
Blondelet-Rouault MH, Weiser J, Lebrihi A, Branny P, Pernodet JL.
|
| タイトル |
Antibiotic resistance gene cassettes derived from the omega interposon for use in E. coli and Streptomyces.
|
| ジャーナル |
Gene
|
| Abstract |
Three antibiotic resistance gene cassettes, derived from the omega interposon (Prentki and Krisch (1984) Gene 29, 303-313) were constructed. These cassettes carry different antibiotic resistance genes, conferring resistance to geneticin, hygromycin or viomycin, flanked by short inverted repeats containing transcription and translation termination signals and synthetic polylinkers. These cassettes were designated omega aac, omega hyg and omega vph. Resistance phenotypes conferred by these constructions are selectable in E. coli and Streptomyces. These cassettes can be used for insertional mutagenesis or for vector construction.
|
| 巻・号 |
190(2)
|
| ページ |
315-7
|
| 公開日 |
1997-5-6
|
| DOI |
10.1016/s0378-1119(97)00014-0
|
| PII |
S0378111997000140
|
| PMID |
9197550
|
| MeSH |
DNA Transposable Elements / genetics*
Drug Resistance, Microbial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics*
Molecular Sequence Data
Streptomyces / drug effects
Streptomyces / genetics*
|
| IF |
2.984
|
| 引用数 |
173
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 原核生物(大腸菌) |
pHP45omega
pHP45omegaaac
pHP45omegahyg |
