RRC ID 33425
著者 Newman JR, Fuqua C.
タイトル Broad-host-range expression vectors that carry the L-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator.
ジャーナル Gene
Abstract We describe the development and analysis of broad-host-range (BHR) cloning vectors that carry the araC-PBAD controlled expression cassette from Escherichia coli. These plasmids are designed to facilitate l-arabinose-responsive control of target genes in a variety of Gram-negative bacterial hosts. BHR PBAD::lacZ fusions were used to analyze the utility of this controlled expression system in the plant pathogen Agrobacterium tumefaciens. In A. tumefaciens, the level of control afforded is significant, although less stringent than that observed in E. coli. The BHR PBAD vectors offer a useful alternative to currently used controlled expression systems, and can be employed in conjunction with other regulated promoters to simultaneously regulate expression of multiple genes. Addition of a variety of carbon sources, namely C4 acids and the anti-inducer d-fucose, allows modulation of l-arabinose induction. Activation of PBAD expression in A. tumefaciens requires a plasmid-borne copy of araC, and is not affected by endogenous regulators.
巻・号 227(2)
ページ 197-203
公開日 1999-2-18
DOI 10.1016/s0378-1119(98)00601-5
PII S0378-1119(98)00601-5
PMID 10023058
MeSH Agrobacterium tumefaciens / genetics* Arabinose / genetics Arabinose / pharmacology* Base Sequence Escherichia coli / genetics* Fucose / pharmacology Gene Expression Regulation, Plant / genetics Genes, Bacterial / genetics Genes, araC / genetics* Genetic Vectors / genetics* Lac Operon / genetics Molecular Sequence Data Plasmids / genetics
IF 2.984
引用数 255
WOS 分野 GENETICS & HEREDITY
リソース情報
原核生物(大腸菌) pCF430 pJN101 pJN105 pJN106