論文 - 詳細
| RRC ID | 33428 |
|---|---|
| 著者 | Amann E, Brosius J, Ptashne M. |
| タイトル | Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. |
| ジャーナル | Gene |
| Abstract |
A strong promoter has been cloned on a series of plasmid vectors that facilitate the expression of cloned genes. This promoter, named tac [first described by DeBoer et al. (in Rodriguez, R.L. and Chamberlin, M.J. (Eds.),Promoters, Structure and Function. Praeger, New York, 1982, pp. pp. 462-481)] contains the -10 region of the lacUV5 promoter and the -35 region of the trp promoter. Our vectors contain various cloning sites followed by transcription termination signals. In addition, we describe plasmids that facilitate the conversion of the lac promoter to the stronger tac promoter. Thus, preexisting gene fusions using the lac or the lacUV5 promoter can be readily converted to tac promoter gene fusions without changing the ribosome-binding site (RBS). The tac promoter is repressed in lacIQ strains and can be induced by isopropylthio-beta-D-galactoside (IPTG). Studies of expression of the cI repressor of bacteriophage lambda show that the tac promoter is at least five times more efficient than the lacUV5 promoter. Under optimal conditions lambda repressor constitutes up to 30% of the total cellular protein. |
| 巻・号 | 25(2-3) |
| ページ | 167-78 |
| 公開日 | 1983-11-1 |
| DOI | 10.1016/0378-1119(83)90222-6 |
| PII | 0378-1119(83)90222-6 |
| PMID | 6363212 |
| MeSH | Bacterial Proteins / analysis Base Sequence Cloning, Molecular DNA, Bacterial / analysis Escherichia coli / genetics* Gene Expression Regulation* Lac Operon* Plasmids* |
| IF | 2.984 |
| 引用数 | 673 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 307 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ptac11 ptac12 ptac12H |