論文 - 詳細
| RRC ID | 33431 |
|---|---|
| 著者 | Brosius J. |
| タイトル | Plasmid vectors for the selection of promoters. |
| ジャーナル | Gene |
| Abstract |
Vectors were constructed which contain promoterless genes for chloramphenicol (cam) or tetracycline (tet) resistance, as promoter-probe plasmids. Escherichia coli cells harboring these plasmids are sensitive to cam or tet but resistant to ampicillin. In plasmids pKK231 -1 and pKK232 -8 the gene for cam acetyltransferase (CAT) and in pKK175 -6 the gene for tet resistance are flanked by efficient transcription terminators, preventing transcription from other pBR322 promoters into the antibiotic resistance region. In one of the vectors, pKK232 -8, translational stop codons were introduced in all three reading frames upstream from the initiation codon of the cat gene. If a DNA fragment containing a promoter is inserted into one of the cloning sites upstream from the antibiotic genes, cells carrying such plasmids acquire resistance to cam or tet. Using these vectors two restriction fragments that contain promoters were identified. One of these fragments contains sequences upstream from an unidentified gene ( ORFII ) located distal to the rrnB rRNA operon of E. coli. |
| 巻・号 | 27(2) |
| ページ | 151-60 |
| 公開日 | 1984-2-1 |
| DOI | 10.1016/0378-1119(84)90136-7 |
| PII | 0378-1119(84)90136-7 |
| PMID | 6327464 |
| MeSH | Acetyltransferases / genetics Bacterial Proteins / genetics Chloramphenicol / pharmacology Chloramphenicol O-Acetyltransferase Drug Resistance, Microbial Escherichia coli / drug effects Escherichia coli / genetics* Gene Expression Regulation Genetic Vectors* Operon* Plasmids* Protein Biosynthesis Tetracycline / pharmacology |
| IF | 2.984 |
| 引用数 | 430 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pKK232-8 |