論文 - 詳細
| RRC ID | 33434 |
|---|---|
| 著者 | Larsen JE, Gerdes K, Light J, Molin S. |
| タイトル | Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter. |
| ジャーナル | Gene |
| Abstract |
By insertion of a DNA fragment, containing the phage lambda pR promoter and the pM-promoted cI857 allele of the lambda repressor gene, in plasmid R1 upstream of the replication control genes, cloning vectors have been constructed which are present in one copy per chromosome at temperatures below 37 degrees C, and which display uncontrolled replication at 42 degrees C. Derivatives have been made which carry the R1 par region, stabilizing the plasmid at low temperature when grown in the absence of selection pressure. Cells harbouring these plasmids stop growing after 1-2 h incubation at 42 degrees C, and at this time 50% of the total DNA in the cells is plasmid DNA corresponding to more than 1000 plasmid molecules per cell. Concomitant with plasmid amplification at the high temperature, synthesis of plasmid-coded gene products is amplified, and these vectors can therefore be utilized for obtaining greatly enhanced yields of gene products that may be detrimental to the host cell when present in large amounts. |
| 巻・号 | 28(1) |
| ページ | 45-54 |
| 公開日 | 1984-4-1 |
| DOI | 10.1016/0378-1119(84)90086-6 |
| PII | 0378-1119(84)90086-6 |
| PMID | 6329915 |
| MeSH | DNA Replication DNA, Bacterial / biosynthesis DNA, Recombinant / biosynthesis Gene Expression Regulation* Genetic Vectors* Operon Plasmids* Temperature |
| IF | 2.984 |
| 引用数 | 166 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pOU71 |