Reference - Detail
RRC ID | 33436 |
---|---|
Author | Rao RN. |
Title | Construction and properties of plasmid pKC30, a pBR322 derivative containing the pL-N region of phage lambda. |
Journal | Gene |
Abstract |
The phage lambda N gene, contained on a HindIII-BamHI lambda DNA fragment (lambda coordinates 38 695-34 500), was inserted into plasmid pBR322 and cloned in Escherichia coli containing a defective lambda prophage. The plasmid is called pKC30. lambda N-mediated pL transcription interfered with plasmid pKC30 maintenance. Plasmid pKC30 was stabilized (i) by repression of pL, (ii) by a nutL mutation, or (iii) by cloning an "N-unresponsive terminator" sequence downstream from N gene. |
Volume | 31(1-3) |
Pages | 247-50 |
Published | 1984-11-1 |
DOI | 10.1016/0378-1119(84)90216-6 |
PII | 0378-1119(84)90216-6 |
PMID | 6098529 |
MeSH | Bacteriophage lambda / genetics* Cloning, Molecular Escherichia coli / genetics Gene Expression Regulation Genes, Viral* Genetic Vectors* Plasmids* Promoter Regions, Genetic Viral Proteins / genetics* |
IF | 2.984 |
Times Cited | 28 |
WOS Category | GENETICS & HEREDITY |
Resource | |
Prokaryotes E. coli | pKC101 pKC37 |