RRC ID 33437
Author Minton NP.
Title Improved plasmid vectors for the isolation of translational lac gene fusions.
Journal Gene
Abstract The beta-galactosidase fusion vector pMC1403 has been modified to include the unique cloning sites EcoRI, SmaI, BamHI, SalI, AccI, PstI and HindIII. The new vectors (pNM480, pNM481 and pNM482) allow the fusion of genes to beta-galactosidase in all three translational reading frames, and exhibit an increased sensitivity of promoter detection due to a higher copy number.
Volume 31(1-3)
Pages 269-73
Published 1984-11-1
DOI 10.1016/0378-1119(84)90220-8
PII 0378-1119(84)90220-8
PMID 6098531
MeSH Bacterial Proteins / biosynthesis Bacterial Proteins / genetics* Base Sequence Cloning, Molecular DNA Restriction Enzymes Galactosidases / genetics* Genes, Bacterial Genetic Vectors* Plasmids* Promoter Regions, Genetic* Protein Biosynthesis Pseudomonas / genetics beta-Galactosidase / biosynthesis beta-Galactosidase / genetics*
IF 2.984
Times Cited 312
Prokaryotes E. coli pNM481 pNM482