論文 - 詳細
| RRC ID | 33442 |
|---|---|
| 著者 | Bolivar F. |
| タイトル | Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules. |
| ジャーナル | Gene |
| Abstract |
In vitro recombinant DNA techniques were used to construct two new cloning vehicles, pBR324 and pBR235. These vectors, derived from plasmid pBR322, are relaxed replicating elements. Plasmid pBR324 carries the genes from pBR322 coding for resistance to the antibiotics ampicillin (Apr) and tetracycline (Tcr) and the colicin E1 structural and immunity genes derived from plasmid pMBI. Plasmid pBR325 carries the Apr and Tcr genes from pBR322 and the cloramphenicol resistance gene (Cmr) from phage P1Cm. In these plasmids the unique EcoRI restriction site present in the DNA molecule is located either in the colicin E1 structural gene (pBR324) or in the Cmr gene (pBR325). These vectors were constructed in order to have a single EcoRI site located in the middle of a structural gene which when inactivated would allow, for the easy selection of plasmid recombinant DNA molecules. These plasmids permit the molecular cloning and easy selection of EcoRI, BamHI, HindIII, PstI, HincII, SalI, (XamI), Smal, (XmaI), BglII and DpnII restriction generated DNA molecules. |
| 巻・号 | 4(2) |
| ページ | 121-36 |
| 公開日 | 1978-10-1 |
| DOI | 10.1016/0378-1119(78)90025-2 |
| PII | 0378-1119(78)90025-2 |
| PMID | 363519 |
| MeSH | Chromosome Mapping Chromosomes, Bacterial Coliphages / genetics* Coliphages / metabolism DNA Restriction Enzymes DNA, Bacterial* / metabolism DNA, Recombinant / metabolism* Escherichia coli / genetics* Escherichia coli / metabolism Plasmids* Transformation, Bacterial |
| IF | 2.984 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pBR325 |