Reference - Detail
| RRC ID | 33443 |
|---|---|
| Author | Hashimoto-Gotoh T, Kume A, Masahashi W, Takeshita S, Fukuda A. |
| Title | Improved vector, pHSG664, for direct streptomycin-resistance selection: cDNA cloning with G:C-tailing procedure and subcloning of double-digest DNA fragments. |
| Journal | Gene |
| Abstract |
A plasmid cloning vector, pHSG664, has been constructed which is suitable for the direct-selection of transformed cells with recombinant DNAs. The plasmid contains the replicon and ApR-gene region of pUC9 ligated to the strA+ (rpsL+) gene derived from pNO1523 [Dean, Gene 15 (1981) 99-102]. The vector contains ten unique restriction sites: EcoRI, HpaI, PvuII, SphI, PstI, SmaI(XmaI), BamHI, SalI(AccI, HincII), XbaI and HindIII. Five sites (bold-face lettering) are located within the coding region of the strA+ gene. Any insertion at the five bold-faced sites or any nucleotide replacement involving the strA+ gene region and the other unique sites can be selected by Ap and Sm double selection in a strA- (SmR) strain such as E. coli HB101. Thus, this vector is useful for cDNA cloning at either the SphI or the PstI site with the G:C-tailing procedure, as well as for the cloning of double-digest DNA segments. |
| Volume | 41(1) |
| Pages | 125-8 |
| Published | 1986-1-1 |
| DOI | 10.1016/0378-1119(86)90275-1 |
| PII | 0378-1119(86)90275-1 |
| PMID | 3009270 |
| MeSH | Base Sequence Cloning, Molecular* DNA / metabolism* DNA Restriction Enzymes Drug Resistance, Microbial Escherichia coli / genetics* Escherichia coli Proteins Genes, Bacterial Genetic Vectors* Ribosomal Protein S9 Selection, Genetic Streptomycin / pharmacology* |
| IF | 2.984 |
| Times Cited | 23 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 34 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | pHSG664 |