| RRC ID |
33445
|
| 著者 |
Wyckoff E, Sampson L, Hayden M, Parr R, Huang WM, Casjens S.
|
| タイトル |
Plasmid vectors useful in the study of translation initiation signals.
|
| ジャーナル |
Gene
|
| Abstract |
The construction and characterization of plasmid vectors useful in the analysis of translation initiation signals in Escherichia coli and in the construction of lacZ gene hybrids are described. Transcription on the vectors proceeds from a cAMP-independent lac promoter through several restriction sites into a truncated lacZ structural gene lacking its first eight codons. Because this gene has no translation initiation signal, its level of expression is extremely low. A DNA fragment containing a translation start signal can be inserted between the promoter and truncated lacZ gene to produce a hybrid protein with functional beta-galactosidase activity. The vectors described here differ in sequence between the EcoRI cloning site and the lacZ gene to allow easy, in-frame joining of DNA containing a translation initiation signal to the lacZ gene. Cells containing plasmids can be screened directly for in-frame inserts by colony color on indicator plates.
|
| 巻・号 |
43(3)
|
| ページ |
281-6
|
| 公開日 |
1986-1-1
|
| DOI |
10.1016/0378-1119(86)90217-9
|
| PII |
0378-1119(86)90217-9
|
| PMID |
3091457
|
| MeSH |
Genetic Vectors*
Lac Operon
Peptide Chain Initiation, Translational*
Plasmids
Promoter Regions, Genetic
beta-Galactosidase / genetics
|
| IF |
2.984
|
| 引用数 |
11
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 原核生物(大腸菌) |
pTSV-1
pTSV-2a
pTSV-2b
pTSV-3a
pTSV-3b |
