論文 - 詳細
| RRC ID | 33449 |
|---|---|
| 著者 | Oberbäumer I. |
| タイトル | New pUC-derived expression vectors for rapid construction of cDNA libraries. |
| ジャーナル | Gene |
| Abstract |
We have constructed a new series of the pUC-derived plasmids with an extended polylinker obtained from M13tg131 [Kieny et al., Gene 26 (1983) 91-99]. These vectors allowed us to design a simplified version of the method of Okayama and Berg [Mol. Cell. Biol. 2 (1982) 161-170] for establishing complete cDNA libraries. Improvements included easy recovery of the inserted cDNA due to the extended polylinkers; use of these vectors for gene expression in Escherichia coli, and amenability to supercoil sequencing with the universal primers of the M13 system [Chen and Seeburg, DNA 4 (1985) 165-170], which speeds up the identification of positive clones. Moreover, there is no need for an additional linker fragment, as was required by the Okayama and Berg [Mol. Cell. Biol. 2 (1982) 161-170] method. We have successfully used this system to obtain cDNA clones coding for the different chains of the large basement membrane proteins type IV collagen and laminin. |
| 巻・号 | 49(1) |
| ページ | 81-91 |
| 公開日 | 1986-1-1 |
| DOI | 10.1016/0378-1119(86)90387-2 |
| PII | 0378-1119(86)90387-2 |
| PMID | 2436975 |
| MeSH | Amino Acid Sequence Base Sequence Cell Line Cloning, Molecular* DNA / metabolism* DNA Restriction Enzymes Female Genes Genetic Vectors* Humans Placenta Plasmids RNA / isolation & purification |
| IF | 2.984 |
| 引用数 | 42 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pOM1 pOM2 pOM3 pOM4 pOM8 pOM9 pUC830 pUC931 |