論文 - 詳細
| RRC ID | 33459 |
|---|---|
| 著者 | Takeshita S, Sato M, Toba M, Masahashi W, Hashimoto-Gotoh T. |
| タイトル | High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection. |
| ジャーナル | Gene |
| Abstract |
Three types of alpha-complementation plasmid vectors were constructed which contain a chloramphenicol- or kanamycin-resistance (CmR or KmR) gene and polylinker cloning sites within the coding region of lacZ'. These vectors are essentially based on high- or low-copy-number replicons. The low-copy-number vectors, 3.61 kb in size, confer CmR and contain the pSC101 replicon and pUC8-/pUC9-type polylinker. On the other hand, the high-copy-number vectors, 2.21 to 2.68 kb in size, confer either CmR or KmR, and contain the pBR322 replicon and pUC18-/pUC19-type or other modified polylinkers. All cloning sites except HindIII and SmaI sites in the KmR vectors are unique in each plasmid. Since almost all frequently used plasmid vectors confer ampicillin resistance, these vectors may be useful to simplify the subcloning/DNA joining experiments due to unnecessity of radioisotope labelling, size fractionation and purification of foreign DNA segments. |
| 巻・号 | 61(1) |
| ページ | 63-74 |
| 公開日 | 1987-1-1 |
| DOI | 10.1016/0378-1119(87)90365-9 |
| PII | 0378-1119(87)90365-9 |
| PMID | 3327753 |
| MeSH | Base Sequence Chloramphenicol Resistance Chromosome Mapping Cloning, Molecular DNA, Bacterial / genetics Escherichia coli / genetics Gene Amplification Genetic Complementation Test Genetic Vectors Kanamycin Resistance Lac Operon* Molecular Sequence Data R Factors* |
| IF | 2.984 |
| 引用数 | 673 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 41 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 原核生物(大腸菌) | pHSG298 pHSG299 pHSG396 pHSG397 pHSG398 pHSG399 pHSG415r pHSG575 pHSG576 pHSG587 |